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Analysis of BRCA2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2499-LGZ)

Introduction

Official Full Name: BRCA2 DNA repair associated<br />Also known as: FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2<br />Inherited mutations in the BRCA1 and BRCA2 genes increase the lifetime risk of breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in the maintenance of genome stability, especially the homologous recombination pathway of double-strand DNA repair. The largest exon of these two genes is exon 11, which contains the most important and most common mutations in breast cancer patients. The BRCA2 gene is located on human chromosome 13q12.3. The BRCA2 protein contains several copies of a 70 aa motif, called the BRC motif, that mediate binding to the RAD51 recombinase, which functions in DNA repair. BRCA2 is considered a tumor suppressor gene because tumors with BRCA2 mutations often exhibit loss of heterozygosity (LOH) for the wild-type allele.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements