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Analysis of BSA adsorption onto nanospheres by UV-Vis Spectroscopy (CAT#: STEM-MB-0981-WXH)

Introduction

Understanding protein adsorption to colloidal particles is of great practical interest and has broad industrial applications. In biomedicine, for instance, controlling protein adsorption to natural and synthetic colloids is crucial for the development of drug-delivery systems, immunological assays, artificial tissues and organs, and biosensing devices. In environmental science, accurate knowledge about protein adsorption to soil particles is an essential prerequisite for, for example, assessing the stability and environmental fate of transgene proteins released by genetically modified crops. As such, the mechanisms of protein adsorption to microscopic colloidal particles have been the subject of many investigations.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
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