Analysis of C-Peptide (Human, Non-human primate) by ELISA (CAT#: STEM-MB-0758-LGZ)

Introduction

C-peptide, also known as connecting peptide, is secreted by pancreatic β-cells and has a common precursor, proinsulin, with insulin. Proinsulin is split into 1 molecule of insulin and 1 molecule of C-peptide, so the molarity of C-peptide is the same as that of self-insulin. Because C-peptide is not easily degraded by the liver, the measurement of C-peptide is the measurement of insulin content, which can accurately reflect the function of islet cells. For patients receiving insulin therapy, measuring insulin levels in the blood cannot evaluate their own islet function, and C-peptide levels can be used to evaluate their own islet β-cell function.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cit plasma, Hep Plasma, Serum

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