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Analysis of C-Peptide (Human, Non-human primate) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0120-LGZ)

Introduction

C-peptide, also known as connecting peptide, is secreted by pancreatic β-cells and has a common precursor, proinsulin, with insulin. Proinsulin is split into 1 molecule of insulin and 1 molecule of C-peptide, so the molarity of C-peptide is the same as that of self-insulin. Because C-peptide is not easily degraded by the liver, the measurement of C-peptide is the measurement of insulin content, which can accurately reflect the function of islet cells. For patients receiving insulin therapy, measuring insulin levels in the blood cannot evaluate their own islet function, and C-peptide levels can be used to evaluate their own islet β-cell function.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Mouse Monoclonal
Recombinant Standards: Synthetic peptide

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Cell Culture Supernatant, P800 Plasma, Serum
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