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Analysis of C4BPA Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0819-LGZ)

Introduction

Official Full Name: complement component 4 binding protein alpha
Also known as: PRP; C4BP
This gene encodes a member of a protein superfamily consisting mainly of short consensus repeats arranged in tandem of approximately 60 amino acids. In addition to a unique β-chain, seven identical α-chains encoded by this gene assemble into the major subtype of C4B-binding protein, a multimeric protein that controls the activation of the complement cascade through the classical pathway. The genes encoding the α- and β-chains are located adjacent to each other in the complement activation regulatory gene cluster on human chromosome 1. Two pseudogenes of this gene were also found in the cluster.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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