Analysis of CA14 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0817-LGZ)

Introduction

Official Full Name: carbonic anhydrase 14
Also known as: CAXiV
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They exhibit wide diversity in tissue distribution and subcellular localization. CA XIV is predicted to be a type I membrane protein with the highest sequence similarity to the other transmembrane CA isoform, CA XII; however, they have different tissue-specific expression patterns and thus may exert different physiological roles.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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