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Analysis of Calbindin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0073-LGZ)

Introduction

Calbindin is a general term for a variety of special proteins with the ability to bind calcium ions, which are specific, high-affinity or high-affinity proteins that can reversibly bind calcium. For example, intestinal absorption of calcium and calcium-binding proteins, kidney absorption of calcium and calcium-binding proteins, muscle-binding calcium proteins, calmodulin that regulates metabolism, and even amylase and thermophilic bacteria proteins. The main biological function is to participate in the transport of calcium, which also promotes the absorption of calcium.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Kidney Injury, Toxicology

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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