Analysis of CAMK1D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2080-LGZ)

Introduction

Official Full Name: calcium/calmodulin dependent protein kinase ID<br />Also known as: CKLiK; CaM-K1; CaMKID<br />This gene is a member of the calcium/calmodulin-dependent protein kinase 1 family, a subfamily of serine/threonine kinases. The encoded protein is a component of the calcium-regulated calmodulin-dependent protein kinase cascade. It is involved in multiple processes such as regulation of granulocyte function, activation of CREB-dependent gene transcription, aldosterone synthesis, differentiation and activation of neutrophils, and apoptosis of erythroleukemia cells. Alternative splicing transcript variants encoding different isoforms of this gene have been described.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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