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Analysis of cAMP (Human, Mouse, Rat) by ELISA (CAT#: STEM-MB-0713-LGZ)

Introduction

Cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate are very important second messenger molecules in the process of regulating intracellular energy metabolism by acting on specific protein kinases. Both are abundant in the central nervous system, with AMP involved in higher cortical functions and yclic guanosine monophosphate involved in phototransduction. Most studies suggest that normal adult lumbar spinal fluid contains 15-30 nmol/L of cAMP, with one study reporting that intraventricular levels may be 2-3 times higher than lumbar levels. cAMP, a derivative of adenosine triphosphate (ATP), mediates cAMP-dependent signal transduction pathways in cells of many different organisms. cAMP participates in the transmembrane regulation mechanism of cell metabolism, differentiation and proliferation, and has good cell inhibition and homeostasis regulation in malignant growth.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Intracellular Signaling, GPCR-ligand binding proteins

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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