Analysis of CAPN9 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0840-LGZ)

Introduction

Official Full Name: calpain 9
Also known as: GC36; nCL-4
Calpains are a ubiquitous, conserved family of calcium-dependent cysteine proteases. The calpain protein is a heterodimer composed of an invariant small subunit and a variable large subunit. The large subunit has a cysteine protease domain and both subunits have calcium-binding domains. Calpains have been implicated in neurodegenerative processes because their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is mainly expressed in the stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be involved in stomach cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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