Analysis of Carbonic Anhydrase XII/CA12 by ELISA (CAT#: STEM-MB-1570-LGZ)

Introduction

Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze reversible hydration of carbon dioxide. Carbonic anhydrases are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorbtion, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrase 12 (CA12) is a type I membrane protein that is highly expressed in normal tissues such as the kidney, colon, and pancreas, and overexpressed in 10% of renal clear cell carcinomas. Two transcription variants encoding different isomers of CA12 have been identified. Since sweating depletes salt, the researchers believe the mutation in CA12 is linked to an autosomal recessive condition called hyponatremia.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Hyponatremia

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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