Analysis of Cathepsin L2 (Human) by ELISA (CAT#: STEM-MB-0718-LGZ)

Introduction

Cathepsins are a class of proteases found in the cells of various animal tissues (especially the lysosome), and are the main members of the cysteine protease family. More than 20 species have been found in the biological world, and 11 mainly exist in the human body. They are closely related to many major diseases such as human tumors, osteoporosis, and arthritis, and are a class of target proteases that have attracted much attention in recent years.
Cathepsin L2, (EC 3.4.22.43, also known as cathepsin V or cathepsin U), is a protein encoded in humans by the CTSV gene. The protein is a human cysteine cathepsin, a lysosomal cysteine protease with endopeptidase activity.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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