Analysis of CCL16 / HCC-4 / NCC-4 / LEC by ELISA (CAT#: STEM-MB-1551-LGZ)

Introduction

Chemokine CC ligand 16 (CCL16) is a small molecule cytokine in the CC chemokine family, also known as NCC-4, liver expression chemokine (LEC), and lymphocyte and monocyte chemokine (LMC). This chemokine is expressed by the liver, thymus and spleen and is chemotactic to monocytes and lymphocytes. The expression of CCL16 can be strongly induced by IL-10, IFN-γ and bacterial lipopolysaccharide in monocytes. In addition, CCL16 induces its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5, and CCR8. The chemokine can inhibit the rapid proliferation of bone marrow progenitor cells.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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