Analysis of CD244 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0826-LGZ)

Introduction

Official Full Name: CD244 molecule
Also known as: 2B4; NAIL; Nmrk; NKR2B4; SLAMF4
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of HEATR5B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1816-LGZ)
Analysis of TH2LCRR Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1857-LGZ)
Analysis of MRPL24 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0474-LGZ)
Analysis of ADCY10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0770-LGZ)
Analysis of SNED1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1669-LGZ)
Analysis of Rhipicentor Spp. by Real-Time PCR Method (CAT#: STEM-MB-3691-LGZ)
Analysis of SCN1A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1488-LGZ)
Analysis of Haemophilus Somnus by Real-Time PCR Method (CAT#: STEM-MB-2986-LGZ)