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Analysis of CD31/PECAM-1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0088-LGZ)

Introduction

CD31 is also known as Platelet endothelial cell adhesion molecule-1 (PECAM-1), with a molecular weight of 130kDa. The structure of CD31 belongs to the immunoglobulin superfamily and plays an important role in clearing aging neutrophils from the body. CD31 is found on the surface of platelets, neutrophils, monocytes, and some types of T cells, as well as in tight junctions between endothelial cells. It may be involved in leukocyte migration, angiogenesis and integrin activation.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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