Analysis of CD46 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0856-LGZ)

Introduction

Official Full Name: CD46 molecule<br />Also known as: MCP; TLX; AHUS2; MIC10; TRA2.10<br />The protein encoded by this gene is a type I membrane protein that is a regulatory part of the complement system. The encoded protein has cofactor activity and can inactivate complement components C3b and C4b through serum factor I, thereby protecting host cells from complement damage. In addition, the encoded protein acts as a receptor for measles virus Edmonston strain, human herpesvirus-6, and pathogenic Neisseria type IV pili. Finally, the protein encoded by this gene may be involved in the fusion of sperm and oocyte during fertilization. Mutations at this site are associated with susceptibility to hemolytic uremia. Alternately spliced transcript variants encoding different isoforms have been described.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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