Analysis of CDH23 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2075-LGZ)

Introduction

Official Full Name: cadherin related 23
Also known as: PITA5; USH1D; CDHR23
This gene is a member of the cadherin superfamily, whose genes encode calcium-dependent cell-cell adhesion glycoproteins. The encoded protein is thought to be involved in the organization of cilia and the formation of hair bundles. This gene is located in a region that includes the human deafness genes DFNB12 and USH1D. Usher syndrome 1D and nonsyndromic autosomal recessive deafness DFNB12 are caused by allelic mutations in this cadherin-like gene. Upregulation of this gene may also be associated with breast cancer. Alternative splice variants encoding different isoforms have been described.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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