Analysis of CDHR5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2234-LGZ)

Introduction

Official Full Name: cadherin related family member 5
Also known as: MLPCDH; MUCDHL; MUPCDH; MU-PCDH
This gene is a novel mucin-like gene that is a member of the cadherin superfamily. While encoding nonpolymorphic tandem repeats rich in proline, serine and threonine similar to mucin proteins, the gene also contains sequence encoding calcium-binding motifs found in all cadherins. The role of the hybrid extracellular region and the specific function of this protein have not yet been determined. Alternatively spliced transcript variants encoding different isoforms have been described.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Covert Mortality Nodavirus (CMNV) by Real-Time PCR Method (CAT#: STEM-MB-3290-LGZ)
Analysis of ATP1A1-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0133-LGZ)
Analysis of JUP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2483-LGZ)
Analysis of Aphanomyces Invadans by Real-Time PCR Method (CAT#: STEM-MB-3857-LGZ)
Analysis of Inini Virus by Real-Time PCR Method (CAT#: STEM-MB-3607-LGZ)
Miniprimer PCR (CAT#: STEM-MB-0213-WXH)
Analysis of Proteus Vulgaris by Real-Time PCR Method (CAT#: STEM-MB-3798-LGZ)
Analysis of Cytomegalovirus (CMV) by Real-Time PCR Method (CAT#: STEM-MB-2593-LGZ)