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Analysis of CEACAM-5 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0100-LGZ)

Introduction

Carcinoembryonic antigen (CEA), also known as CD66e and CEACAM5, is a glycoprotein with a molecular weight of 180-200kD involved in cell adhesion. CEA is usually produced in the fetal digestive system, but because it is suppressed after birth, the content of CEA in the colon and blood of normal adults is usually very low. However, elevated serum levels of CEA can be caused by various types of cancer including colorectal, lung, and breast cancers. Therefore, CEA is widely used as a broad-spectrum tumor marker. CEA is not specific for any kind of cancer, but the concentration of CEA in blood can predict the therapeutic effect, progress and prognosis of cancer.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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