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Analysis of CENPF Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0848-LGZ)

Introduction

Official Full Name: centromere protein F
Also known as: CENF; hcp-1; CILD31; STROMS; PRO1779
This gene encodes a protein that binds to the centromere-centromere complex. This protein is a component of the nuclear matrix in interphase G2 phase. During late G2, the protein binds to the kinetochore and maintains this binding through early anaphase. It is located in the anaphase and telophase of the spindle mesozone and intracellular bridge, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitosis. It is thought to form homodimers or heterodimers. Autoantibodies against this protein are found in patients with cancer or graft-versus-host disease.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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