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Analysis of CENPS Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1283-LGZ)

Introduction

Official Full Name: centromere protein S
Also known as: MHF1; APITD1; CENP-S; FAAP16
The gene is located in a neuroblastoma tumor suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain similar to that found in the TATA box-binding protein-associated factor TAF(II)31, which is required for p53-mediated transcriptional activation. The gene is expressed at very low levels in neuroblastoma tumors and has been shown to reduce cell growth in neuroblastoma cells, suggesting that it may play a role in the cell death pathway. This protein is part of several complexes, including the Fanconi anemia (FA) core complex, the APITD1/CENPS complex, and the CENPA-CAD (distal nucleosome) complex. Known functions include participation in chromatin binding of the FA core complex and a role in the stable assembly of the outer centromere. Alternative splicing of this gene results in multiple transcript variants. There is also a native readable transcript between this gene and the downstream cortistatin (CORT) gene. A pseudogene associated with apitd1 was found on chromosome 7.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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