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Analysis of CEP170 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0846-LGZ)

Introduction

Official Full Name: centrosomal protein 170<br />Also known as: KAB; FAM68A; KIAA0470<br />The product of this gene is a component of the centrosome, a non-membrane organelle that functions as the main microtubule organizing center in animal cells. During interphase, the encoded protein localizes to the subdistal appendages of mature centrioles, microtubule structures thought to help organize centrosomes. During mitosis, the protein binds to spindle microtubules near the centrosome. This protein interacts with and is phosphorylated by polo-like kinase 1, which plays a role in maintaining microtubule organization and cell morphology. The human genome contains a putative transcriptional pseudogene. Several alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements