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Analysis of Charge and potential of cells/membranes by Electron paramagnetic resonance (EPR) spectroscopy (CAT#: STEM-MB-1041-WXH)

Introduction

All living cells maintain a potential difference across their membrane. Simply stated, membrane potential is due to disparities in concentration and permeability of important ions across a membrane. Because of the unequal concentrations of ions across a membrane, the membrane has an electrical charge.
Changes in membrane potential elicit action potentials and give cells the ability to send messages around the body. More specifically, the action potentials are electrical signals; these signals carry efferent messages to the central nervous system for processing and afferent messages away from the brain to elicit a specific reaction or movement. Numerous active transports embedded within the cellular membrane contribute to the creation of membrane potentials, as well as the universal cellular structure of the lipid bilayer.




Principle

Electron Paramagnetic Resonance (EPR), also called Electron Spin Resonance (ESR), is a branch of magnetic resonance spectroscopy which utilizes microwave radiation to probe species with unpaired electrons, such as radicals, radical cations, and triplets in the presence of an externally applied static magnetic field.
EPR spectroscopy is particularly suitable for the investigation of (bio)chemical systems with strongly localized spin density and their interaction with the environment. For these systems EPR provides information on the structure and dynamics and is widely used in chemistry, physics and biology.

Applications

• Study dynamic organisation of lipids in biological membranes, lipid-protein interactions and temperature of transition of gel to liquid crystalline phases.
• Determine oxygen levels in tissues and blood.
• Injection of spin-labeled molecules allows for electron resonance imaging of living organisms.
• EPR can be used to measure microviscosity and micropolarity within drug delivery systems as well as the characterization of colloidal drug carriers.
• The study of radiation-induced free radicals in biological substances (for cancer research).
• Investigation on the antioxidant properties of medicine

Procedure

1. Sample Preparation
2. Electron paramagnetic resonance (EPR) spectroscopy testing
5. Data analysis

Materials

• EPR Spectrometer
• Spectrophotometer
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