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Analysis of Chitosan-Chromone Derivative by UV-Vis Spectroscopy (CAT#: STEM-MB-0931-WXH)

Introduction

Chitosan (CS) is the second-most abundant biopolymer derived from the Ndeacetylation of chitin. The design and synthesis of chitosan-based materials has been attracted by researcher groups due to their unique biological and physicochemical properties such as biocompatibility, biodegradability and film-forming ability.
Chromones (4H-chromone-4-ones) are heterocyclic compounds with the benzo-γ-pyrone framework. Chromones and their structural analogs are of great interest because of their usefulness as biologically active agents. They have been shown to be tyrosine and protein kinase inhibitors, as well as anticancer, neuroprotective, anti-AIDS, antimicrobial, antifungal, antioxidant activity, interleukin-5 inhibitory activity, and antiproliferative agents.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
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