Analysis of Chlamydomonas Reinhardtii BafJ5 by RT-qPCR (CAT#: STEM-MT-0053-LGZ)

Introduction

Carbon sources are key factors in lipid biosynthesis in microalgae. The supply of exogenous inorganic carbon or organic carbon influences lipid accumulation in microalgae under stress conditions. However, the effect of different carbon availability on glycerolipid metabolism, especially triacylglycerol (TAG) metabolism in microalgae remains unknown. Chlamydomonas starch-free mutant BAFJ5 is a model system for studying TAG metabolism due to its hyperaccumulation of TAG.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Mutation Analysis

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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