Analysis of Choline Acetyltransferase (ChAT) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1427-LGZ)

Introduction

Sensitivity: 1.21 U/g
Detection range: 1.21-40 U/g
Precision: inter-lot difference of 9.4%, intra-lot difference of 5.1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 324 nm

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Acetylcholintransferase (ChAT) takes acetyl-coA and choline as substrate to produce CoA (CoA). CoA combines with color developing agent 4, 4-dithiopyridine. Absorbance is measured at 324nm to calculate the activity of ChAT.

Applications

Used to detect ChAT activity in various animal tissues.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: various animal tissues

Other recommended products

Analysis of Cell Ferrous Iron by Enzyme-labeled Instrument (CAT#: STEM-MB-1462-LGZ)
Analysis of Total Antioxidant Capacity (T-AOC) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1326-LGZ)
Analysis of Catalase (CAT) Activity by Fluorescence Microplate Reader (CAT#: STEM-MB-1501-LGZ)
Analysis of Pyruvic Acid by Enzyme-labeled Instrument (CAT#: STEM-MB-1404-LGZ)
Analysis of Pyruvic Acid by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1336-LGZ)
Analysis of Glucose (Glu) by Enzyme-labeled Instrument (CAT#: STEM-MB-1376-LGZ)
GC-MS-based Untargeted Metabolomics for high-throughput analysis (CAT#: STEM-MB-0105-WXH)
Analysis of Non-esterified Free Fatty Acids (NEFA/FFA) by Enzyme-labeled Instrument (CAT#: STEM-MB-1499-LGZ)