Analysis of CHTOP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0864-LGZ)

Introduction

Official Full Name: chromatin target of PRMT1<br />Also known as: FOP; SRAG; SRAG-3; SRAG-5; pp7704; C1orf77; FL-SRAG; C10orf77<br />This gene encodes a small nucleoprotein characterized by an arginine- and glycine-rich region. This protein may play an important role in regulating fetal globin gene expression and activating estrogen-responsive genes. A recent study reported that this protein binds 5-hydroxymethylcytosine (5hmC) and associates with an arginine methyltransferase complex (methylosome), which promotes methylation of arginine 3 of histone H4 (H4R3) and activation of genes involved in glioblastom agenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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