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Analysis of cIAP1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0106-LGZ)

Introduction

The inhibitor of apoptosis protein (IAP) family comprises a group of evolutionarily conserved inhibitors of apoptosis that contain a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain. Human protein members of this family include c-IAP1, c-IAP2, XIAP, surviving, livin, and NAIP. Members of the IAP family are overexpressed in cancer cell lines and primary tumors, and these proteins play an important role in cancer progression.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma
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