Analysis of CNGA3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1446-LGZ)

Introduction

Official Full Name: cyclic nucleotide gated channel subunit alpha 3 <br />Also known as: CNG3; ACHM2; CCNC1; CCNCa; CNCG3; CCNCalpha<br />This gene encodes a member of the cyclic nucleotide-gated cation channel protein family which is required for normal vision and olfactory signal transduction. Mutations in this gene are associated with achromatopsia (rod monochromacy) and color blindness. Two alternatively spliced transcripts encoding different isoforms have been described.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of LOC115947637 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1606-LGZ)
Analysis of Cryptococcus Spp. by Real-Time PCR Method (CAT#: STEM-MB-2606-LGZ)
Analysis of Eperythrozoon Suis by Real-Time PCR Method (CAT#: STEM-MB-3898-LGZ)
Analysis of Burkholderia Pseudomallei by Real-Time PCR Method (CAT#: STEM-MB-2721-LGZ)
Analysis of SYCP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1165-LGZ)
Analysis of IMMT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1677-LGZ)
Analysis of RPS20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1869-LGZ)
Analysis of RBP7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1400-LGZ)