Analysis of CNOT7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1921-LGZ)

Introduction

Official Full Name: CCR4-NOT transcription complex subunit 7
Also known as: CAF1; CAF-1; Caf1a; hCAF-1
The protein encoded by this gene binds to an anti-proliferation protein B-cell translocation protein 1, and negatively regulates cell proliferation. The association of these two proteins is driven by the phosphorylation of anti-proliferative proteins, causing signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contacts. The encoded protein downregulates the innate immune response, thus providing a therapeutic target to enhance its antimicrobial activity against foreign pathogens. Alternative splicing of this gene results in multiple transcript variants. Associated pseudogenes were found on chromosomes 1 and X.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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