Analysis of CNTF by ELISA (CAT#: STEM-MB-1566-LGZ)

Introduction

Ciliary neurotrophic factor (CNTF) is a protein encoded by the ciliary neurotrophic factor gene in humans. Human CNTF is a single-stranded polypeptide with 200 amino acid (aa) residues and a molecular weight of 22.7 kDa. The protein encoded by the gene is a polypeptide hormone whose role is limited to promoting the synthesis of neurotransmitters in the nervous system and the axonal growth of certain groups of neurons. CNTF is a potent survival factor for neurons and oligodendrocytes and may be involved in reducing tissue destruction during inflammatory episodes. It has been shown that CNTF can also be expressed in bone surface cells and reduce the activity of osteoblasts.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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