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Analysis of COL4A4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1526-LGZ)

Introduction

Official Full Name: collagen type IV alpha 4 chain<br />Also known as: BFH; ATS2; BFH1; CA44<br />The gene encodes one of the six subunits of type IV collagen, the main structural component of the basement membrane. However, this particular collagen IV subunit is only present in one subunit of the basement membrane. Like other members of the collagen IV gene family, this gene is in a head-to-head conformation with another collagen IV gene, so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and familial benign hematuria (thin basement membrane disease). Two transcripts of this gene have been identified, differing only in the transcription start site, and like the collagen gene, multiple polyadenylation sites were found in the 3' UTR.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Alport syndrome

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements