Analysis of CREG1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0876-LGZ)

Introduction

Official Full Name: cellular repressor of E1A stimulated genes 1
Also known as: CREG
Adenovirus E1A protein activates and represses gene expression, promotes cell proliferation and inhibits differentiation. The protein encoded by this gene can antagonize the transcriptional activation and cell transformation of E1A. This protein shares limited sequence similarity with E1A and binds the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may be involved in the transcriptional control of cell growth and differentiation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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