Analysis of CREG1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0876-LGZ)

Introduction

Official Full Name: cellular repressor of E1A stimulated genes 1<br />Also known as: CREG<br />Adenovirus E1A protein activates and represses gene expression, promotes cell proliferation and inhibits differentiation. The protein encoded by this gene can antagonize the transcriptional activation and cell transformation of E1A. This protein shares limited sequence similarity with E1A and binds the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may be involved in the transcriptional control of cell growth and differentiation.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of BOLA1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0797-LGZ)
Analysis of SERPINC1 Gene (Mutation) in Hereditary Antithrombin Deficiency by RT-qPCR (CAT#: STEM-MT-0166-LGZ)
Analysis of Fusarium Tricinctum by Real-Time PCR Method (CAT#: STEM-MB-3444-LGZ)
Analysis of HES3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1328-LGZ)
Analysis of PRAMEF9 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1387-LGZ)
Analysis of PDCD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1652-LGZ)
Analysis of TMEM43 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1831-LGZ)
Analysis of RIC3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2107-LGZ)