Analysis of CSRP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2464-LGZ)

Introduction

Official Full Name: cysteine and glycine rich protein 3<br />Also known as: CLP; MLP; CRP3; LMO4; CMD1M; CMH12<br />This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in important regulatory processes of development and cell differentiation. The LIM/double zinc finger motif found in this protein is present in a group of proteins with key functions in gene regulation, cell growth and somatic cell differentiation. Mutations in this gene are thought to cause inherited forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternative spliced transcript variants of this gene have been found with different 5' UTRs but encode the same protein.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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