Analysis of CXCR4 Mutation by RT-qPCR (CAT#: STEM-MT-0060-LGZ)

Introduction

Waldenström macroglobulinemia (WM) is a rare lymphoproliferative disorder characterized by the presence of serum IgM paraproteins associated with bone marrow infiltration of lymphoplasmacytic lymphoma.<br />Some years ago, using whole genome sequencing Treon et al. identified MYD88 (L265P) as the most common somatic mutation in WM. The second most common mutations in WM are nonsense or frameshift mutations in the carboxyl-terminal cytoplasmic tail of the CXCR4 gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Waldenström Macroglobulinemia (WM)

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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