Analysis of Cystatin C (Human) by ELISA (CAT#: STEM-MB-0765-LGZ)

Introduction

Cysteine proteinase inhibitor (CP) was named cystatin C. Known as gamma-trace protein and gamma-globulin, widely exist in all kinds of tissue nucleated cells and body fluids, is a low molecular weight, basic non-glycated protein, molecular weight of 13.3KD, composed of 122 amino acid residues, can be produced by the body all nucleated cells, the production rate is constant. Circulating cystatin c is cleared only by glomerular filtration, an endogenous marker reflecting changes in glomerular filtration rate. It is reabsorbed in the proximal curving tubule, but is completely metabolized after reabsorption and does not return to the blood. Therefore, its blood concentration is determined by glomerular filtration rate, independent of any external factors such as sex, age, and diet. It is an ideal endogenous marker to reflect the change of glomerular filtration rate.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Serum, Urine

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