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Analysis of DCTN1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1790-LGZ)

Introduction

Official Full Name: dynactin subunit 1
Also known as: P135; DP-150; DAP-150
This gene encodes the largest subunit of dynactin, a macromolecular complex of 10 subunits ranging in size from 22 to 150 kd. Dynein associates with microtubules and cytoplasmic dynein. Dynactin is involved in a variety of cellular functions, including endoplasmic reticulum to Golgi transport, centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear localization, and axonogenesis. This subunit interacts with the dynein middle chain, and its domains directly bind dynein and microtubules through its highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain at its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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