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Analysis of DEDD Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0907-LGZ)

Introduction

Official Full Name: death effector domain containing
Also known as: DEFT; KE05; DEDD1; FLDED1; DEDPro1; FLDED-1; CASP8IP1
This gene encodes a protein that contains a death effector domain (DED). DED is a protein-interaction domain shared by adaptors, regulators, and executors of the programmed cell death pathway. Overexpression of this gene induces weak apoptosis. Upon stimulation, the protein was found to translocate from the cytoplasm to the nucleus and co-localize with UBTF, an essential factor required for RNA polymerase I transcription in the nucleus. This gene has at least three transcript variants encoding the same protein.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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