Analysis of DFFB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1296-LGZ)

Introduction

Official Full Name: DNA fragmentation factor subunit beta<br />Also known as: CAD; CPAN; DFF2; DFF40; DFF-40<br />Apoptosis is the cell death process that eliminates toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of cells and nuclei and degradation of chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is a substrate of caspase-3, which triggers DNA fragmentation during apoptosis. DFF is activated when DFFA is cleaved by caspase-3. The cleavage fragment of DFFA is separated from DFFB, which is the active component of DFF. DFFB has been found to trigger DNA fragmentation and chromatin condensation during apoptosis. Alternately spliced transcript variants of this gene have been found to encode different isoforms, but the biological validity of some of these variants has not been determined.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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