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Analysis of DISP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0918-LGZ)

Introduction

Official Full Name: dispatched RND transporter family member 1<br />Also known as: DISPA<br />The patterns of cell proliferation and differentiation that lead to the normal development of embryonic structures often depend on the local production of secreted protein signals. Cells surrounding a particular signal source respond in a graded fashion according to the effective concentration of the signal, and this response produces a pattern of cell types that constitute the mature structure. A novel fragment polarity gene, called dispatcher, whose protein product is required for normal Hedgehog (Hh) signaling has been identified in Drosophila. This gene is one of the two human homologues of Drosophila, and based on the sequence identity with its mouse counterpart, the encoded protein may play an important role in the Hh patterning activity of early embryos.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements