Analysis of DNA Content by Flow Cytometry (CAT#: STEM-CBT-0062-WXH)

Introduction

The measurement of DNA content of cells is one of the earliest and major application in flow cytometry and still is one that is extensively used today. DNA content analysis by flow cytometry can provide valuable information about the cell cycle status of individual cells.
DNA content measurement demands high precision because small variations in DNA content can have considerable biological significance.
Precise measurement of DNA content becomes possible thanks to the availability of nucleic acid specific fluorescent dyes and the availability of powerful flow cytometers capable of measuring with great precision fluorescence coming from inside cell nucleus.