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Analysis of DNAI1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2046-LGZ)

Introduction

Official Full Name: dynein axonemal intermediate chain 1<br />Also known as: PCD; DIC1; ICS1; CILD1<br />This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the respiratory ciliary dynein complex. Inner-arm and outer-arm dynein, the bridge between the double-threaded microtubules in the axon, is the force-generating protein responsible for sliding motion in the axon. The intermediate and light chains are thought to form the basis of the dynein arms, help regulate attachment, and may also be involved in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements