Analysis of DPM3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0914-LGZ)

Introduction

Official Full Name: dolichyl-phosphate mannosyltransferase subunit 3, regulatory
Also known as: CDG1O; MDDGB15; MDDGC15
Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a subunit of dolichyl-phosphate mannosyltransferase and acts as a stabilizer subunit of the dolichyl-phosphate mannosyltransferase complex.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of MTX1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0388-LGZ)
Analysis of Enterococcus Faecalis by Real-Time PCR Method (CAT#: STEM-MB-4057-LGZ)
Analysis of Avian Herpesvirus 1 (Gallid Herpesvirus-1, GaHV-1) by Real-Time PCR Method (CAT#: STEM-MB-2866-LGZ)
Analysis of IQGAP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1060-LGZ)
Analysis of GJA8 Gene (Mutation) in Cataract 1 Multiple Types by RT-qPCR (CAT#: STEM-MT-0137-LGZ)
Analysis of Batrachochytrium Dendrobatidis by Real-Time PCR Method (CAT#: STEM-MB-2795-LGZ)
Analysis of Bovine Adenovirus (BAV-3) by Real-Time PCR Method (CAT#: STEM-MB-3225-LGZ)
Analysis of Equine Herpesvirus 2 (EHV-2) by Real-Time PCR Method (CAT#: STEM-MB-3172-LGZ)