Analysis of DPYD Gene (Mutation) in Dihydropyrimidine Dehydrogenase Deficiency by RT-qPCR (CAT#: STEM-MT-0118-LGZ)

Introduction

Also known as: DHP; DPD; DYPD; DHPDHASE<br />The protein encoded by this gene is a pyrimidine catabolic enzyme, which is the initiation factor and rate-limiting factor of uracil and thymine catabolic pathway. Mutations in this gene lead to dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymidine uraciluria, and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants of this gene have been found to encode different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Dihydropyrimidine dehydrogenase deficiency

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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