Analysis of DUSP8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2218-LGZ)

Introduction

Official Full Name: dual specificity phosphatase 8<br />Also known as: HB5; HVH8; HVH-5; C11orf81<br />The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are involved in cell proliferation and differentiation. Different members of the dual-specificity phosphatase family exhibit different substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different patterns of induction of their expression by extracellular stimuli. The gene product inactivates SAPK/JNK and p38, is mainly expressed in adult brain, heart and skeletal muscle, localizes in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless DUSP8 pseudogene is present on chromosome 10q11.2.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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