Analysis of ECEL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1765-LGZ)

Introduction

Official Full Name: endothelin converting enzyme like 1
Also known as: XCE; DA5D; DINE; ECEX
This gene encodes a member of the M13 family of endopeptidases. Members of this family are zinc-containing type II integral-membrane proteins that are important regulators of neuropeptide and peptide hormone activity. Mutations in this gene are associated with autosomal recessive distal arthrogryposis, type 5D. This gene has multiple pseudogenes on chromosome 2. Alternative splicing results in multiple transcript variants encoding different isoforms.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PBX1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0563-LGZ)
Analysis of UDP-glycosyltransferase (UGT) by RT-qPCR (CAT#: STEM-MT-0057-LGZ)
Analysis of SIV Ag by Real-Time PCR Method (CAT#: STEM-MB-2910-LGZ)
Analysis of Duck Enteritis Virus by Real-Time PCR Method (CAT#: STEM-MB-3693-LGZ)
Reverse transcription PCR (RT-PCR) (CAT#: STEM-MB-0187-WXH)
Analysis of RIF1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1476-LGZ)
Analysis of SERGEF Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2434-LGZ)
Analysis of PUSL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1396-LGZ)