Analysis of ECM1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0937-LGZ)

Introduction

Official Full Name: extracellular matrix protein 1<br />Also known as: URBWD<br />This gene encodes a soluble protein involved in endochondral bone formation, angiogenesis and tumor biology. It also interacts with a variety of extracellular and structural proteins to help maintain skin integrity and homeostasis. Mutations in this gene are associated with lipoproteinosis (also known as hyalinosis of the skin and mucous membranes or Urbach-Wiethe disease), which is characterized by widespread thickening of the skin, mucous membranes, and certain internal organs. Alternative splicing transcript variants encoding different isoforms have been described for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Human Rhinovirus 9 by Real-Time PCR Method (CAT#: STEM-MB-3269-LGZ)
Analysis of Cylindrocladium Parasiticum by Real-Time PCR Method (CAT#: STEM-MB-2597-LGZ)
Analysis of Ovine Adenovirus by Real-Time PCR Method (CAT#: STEM-MB-3026-LGZ)
Analysis of Nosema Spp. by Real-Time PCR Method (CAT#: STEM-MB-3789-LGZ)
Analysis of BMAL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2408-LGZ)
Analysis of DISC1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0920-LGZ)
Analysis of COX7A2L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1716-LGZ)
Analysis of LYSMD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0456-LGZ)