Analysis of EGFL7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1995-LGZ)

Introduction

Official Full Name: EGF like domain multiple 7<br />Also known as: NEU1; ZNEU1; VE-STATIN<br />This gene encodes a secreted endothelial cell protein that contains two epidermal growth factor-like domains. The encoded protein may play a role in regulating vasculogenesis. This protein may be involved in the growth and proliferation of tumor cells. Alternate splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Mycobacterium Fortuitum by Real-Time PCR Method (CAT#: STEM-MB-2954-LGZ)
Analysis of European Brown Hare Syndrome Virus (EBHSV) by Real-Time PCR Method (CAT#: STEM-MB-3865-LGZ)
Analysis of DNM3OS Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0915-LGZ)
Analysis of Mycobacterium Tuberculosis (MTB) by Real-Time PCR Method (CAT#: STEM-MB-2937-LGZ)
Analysis of Neisseria Meningitidis Group A by Real-Time PCR Method (CAT#: STEM-MB-3080-LGZ)
Analysis of Cylindrocladium Parasiticum by Real-Time PCR Method (CAT#: STEM-MB-2597-LGZ)
Analysis of Carp Edema Virus (CEV) by Real-Time PCR Method (CAT#: STEM-MB-2677-LGZ)
Analysis of Carbapenem-resistant Klebsiella pneumoniae(R-Kp) by Real-Time PCR Method (CAT#: STEM-MB-2679-LGZ)