Analysis of EGFR (Human) by ELISA (CAT#: STEM-MB-0775-LGZ)

Introduction

EGFR is short for epidermal growth factor receptor gene. DNA extracted from tumor cells can be detected for mutations in this gene. In some cases, DNA shed by tumor cells in the blood can also be tested, potentially informative (liquid biopsy). The EGFR gene gives the cell the instructions to make a special type of protein called a receptor, which sits on the surface of the cell. Receptors recognize and bind to other substances, often resulting in specific effects on the cell. EGFR is one of a group of receptors known as receptor tyrosine kinases that help regulate cell growth, division, survival and death. This regulation is a very complex process.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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