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Analysis of EGFR (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0137-LGZ)

Introduction

EGFR is short for epidermal growth factor receptor gene. DNA extracted from tumor cells can be detected for mutations in this gene. In some cases, DNA shed by tumor cells in the blood can also be tested, potentially informative (liquid biopsy). The EGFR gene gives the cell the instructions to make a special type of protein called a receptor, which sits on the surface of the cell. Receptors recognize and bind to other substances, often resulting in specific effects on the cell. EGFR is one of a group of receptors known as receptor tyrosine kinases that help regulate cell growth, division, survival and death. This regulation is a very complex process.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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